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dc.creatorAchí Araya, María Rosario
dc.creatorMata Jiménez, Leonardoes_ES
dc.creatorSiles Díaz, Xinia
dc.creatorLindberg, Alf A.
dc.date.accessioned2015-06-22T19:42:00Z
dc.date.available2015-06-22T19:42:00Z
dc.date.issued1996
dc.identifier.citationhttp://www.journalofinfection.com/article/S0163-4453%2896%2980021-1/abstractes_ES
dc.identifier.issn0163-4453
dc.identifier.urihttp://hdl.handle.net/10669/14795
dc.descriptionArtículo científico -- Universidad de Costa Rica, Instituto de Investigaciones en Salud. 1996. Este documento es privado debido a limitaciones de derechos de autor.es_ES
dc.description.abstractCulture and immunomagnetic separation-polymerase chain reaction assays (IMS-PCR) were used to isolate and identify Shigella flexneri, S. dysenteriae type I and S. sonnei in faeces from 250 children up to 5 years and from their mothers (n = 143) selected at random from a large urban marginal community of Costa Rica. Children hospitalized because of severe diarrhoea (n = 110) were also studied. Only S. flexneri, mainly serotype 2a, and S. sonnei were found by culture. All specimens in which Shigella was cultured were also positive by the corresponding IMS-PCR. S. flexneri was isolated by culture from 1 (0.7%) mother and 4 (1.6%) community children. S. sonnei was found in 2 (0.8%) children. An additional 12 S. flexneri and four S. sonnei in the community children were found by IMS-PCR. In total, Shigella was cultured from 0.7% of mothers and 2.4% of children. By the IMS-PCR 2% of mothers and 8% of children were positive. S. flexneri was isolated by culture from 14 (12.7%) hospitalized children and S. sonnei from 1 (0.9%). An additional 11 S. flexneri and three S. sonnei were found by IMS-PCR. In total, Shigella was cultured from 13.6% of hospitalized children. By the IMS-PCR 26% of them were Shigella positive. Thus IMS-PCR was more than twice as effective in diagnosing shigellae as culture. Twelve (60%) Shigella positive community children were above 3-years-old and 25% of them were under one year. Seven (35%) of the Shigella positive children had dysenteric and 9 (45%) normal stools. Half of the Shigella infected community children had been weaned before the 3 months of age. By the age of 5 months, 90% of them were already weaned. Seventeen (59%) of the hospitalized Shigella positive children were under 1 year of age. The stools were watery or semiliquid in 13 (45%) and dysenteric in 12 (41%) of them. We conclude that shigellosis is common in Costa Rica and represents an important cause of severe infant diarrhoea requiring hospitalization.es_ES
dc.description.sponsorshipUniversidad de Costa Rica. Instituto de Investigaciones en Saludes_ES
dc.language.isoen_USes_ES
dc.sourceJournal Infection 32 (3): 211-218es_ES
dc.subjectCosta Ricaes_ES
dc.subjectShigellaees_ES
dc.subjectUrbano/ Marginales_ES
dc.subjectDiarrheaes_ES
dc.titleImmunomagnetic separation and PCR detection show Shigellae to be common faecal agents in children from urban marginal communities of Costa Ricaes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.typeArtículo científicoes_ES
dc.identifier.doi10.1016/S0163-4453(96)80021-1
dc.description.procedenceUCR::Investigación::Unidades de Investigación::Ciencias de la Salud::Instituto de Investigaciones en Salud (INISA)es_ES


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