New concepts to improve resolution and sensitivity of molecular cytogenetic diagnostics by multicolor fluorescense in situ hybridization
Azofeifa Navas, Jorge
Speicher, Michael R.
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Background: Routine application of multicolor fluorescence in situ hybridization (M-FISH) technology for molecular cytogenetic diagnostics has been hampered by several technical limitations. First, when using chromosome-specific painting probes, there is a limit in cytogenetic resolution of approximately 2–3 Mb, which can mask hidden structural abnormalities that have a significant clinical effect. Second, using whole chromosome painting probes, intrachromosomal rearrangements cannot be detected and the exact localization of breakpoints is often not possible. Methods: We suggest the use of multiplex-labeled region or locus- specific probes in combination with an optimal probe design to improve the sensitivity and resolution of the M-FISH technology. To allow the application of this assay in routine diagnostics, we developed a multipurpose image analysis system. Results: goldFISH was applied to the study of cryptic translocations in mental retardation patients and to the study of high-resolution breakpoint mapping in non-small cell lung cancer patients. For an individual with mental retardation, who had an apparently normal karyotype by G-banding, we detected an unbalanced translocation involving chromosomes 2 and 7. Conclusions: In combination with optimally designed probe kits, goldFISH overcomes most of the present limitations of the M-FISH technology and results in virtually 100% reliability for detecting interchromosomal and intra-chromosomal rearrangements.
External link to the itemDOI:10.1002/1097-0320(20010501)44:1<7::AID-CYTO1076>3.0.CO;2-G
Artículo científico -- Universidad de Costa Rica, Instituto de Investigaciones en Salud, 2001. Este documento es privado debido a limitaciones de derechos de autor.